贵州野生匍匐剪股颖种质资源遗传多样性的ISSR分析

草业学报 ›› 2009, Vol. 18 ›› Issue (3): 67-73.

贵州野生匍匐剪股颖种质资源遗传多样性的ISSR分析

尚以顺¹,杨春燕², 钟理^1*

1.贵州省草业研究所,贵州独山 558200;
2.都匀市农业局,贵州都匀　558000

收稿日期:2008-11-20 出版日期:2009-06-20 发布日期:2009-06-20
作者简介:尚以顺(1969-),男,贵州岑巩人,副研究员。E-mail:gzsyishun@vip.com.com
基金资助:
贵州省草业工程技术中心建设项目]黔科合农字(2005)4002号],贵州省省长基金项目]合同号:黔省专合字(2006)87号]和贵州省“十一五”攻关项目]黔科合NY字(2008)3047号]资助。

Genetic diversity identification based on ISSR analysis of Agrostis stolonifera
germplasm resources in Guizhou

SHANG Yi-shun¹, YANG Chun-yan², ZHONG Li¹

1.Guizhou Prataculturale Institute, Dushan 558200,China;
2.Agricultural
Bureau of Duyun, Duyun 558000,China

Received:2008-11-20 Online:2009-06-20 Published:2009-06-20

摘要/Abstract

摘要： 匍匐剪股颖是我国重要的冷季型草坪草之一。为了研究贵州野生匍匐剪股颖居群遗传变异水平、遗传结构及亲缘关系,本试验采用ISSR标记技术对贵州的4个匍匐剪股颖居群共285个个体进行了遗传变异分析。 9个引物共扩增出81条带,其中多态性条带66条,无论是在居群水平还是在物种水平,均表明贵州野生匍匐剪股颖居群存在丰富的遗传变异;3个野生居群间遗传分化系数(Gst)为0.492 0,居群间基因流的估测值(Nm)为0.516 4,表明居群间存在较为严重的遗传分化;亚居群间的遗传距离(GD)为0.087 7~0.592 6,遗传距离(GD)的聚类分析结果与居群地理分布存在密切联系。利用SPSS 11.0软件对地理距离与亚居群间遗传距离相关性进行分析,表明地理距离与遗传距离呈显著正相关(r=0.494);用POPGENE 1.31软件对居群间的遗传关系分析表明,各居群间的遗传一致度比较高,分布为0.700 3~0.940 9,但栽培居群(KROMI)与其他3个野生居群存在明显的遗传差异。

Abstract: Agrostis stolonifera is a widely grown, cool season turf grass in China. To determine genetic diversity and relationships of A. stolonifera resources, 285 plants from three wild populations and a cultivated population in Guizhou were screened by inter-simple sequence repeat(ISSR)markers. Nine of 60 primer pairs produced a total of 81 bands, of which 66 were polymorphic. Statistic analysis showed a rich genetic diversity of A. stolonifera resources in Guizhou province. The genetic differentiation coefficient (Gst) and the gene flow (Nm)among three wild populations were 0.492 0 and 0.516 4 .The genetic differentiation was considerable in the three wild populations. The genetic distance GD)of the subpopulations ranged from 0.087 7 to 0.592 6. The subpopulations could be clustered into three groups based on GD. Correlation analysis found a significant positive correlation between geographical distance and genetic distance (r=0.494), and this is persuasive evidence that there is a significant correlation between genetic component and the geographic distribution of A. stolonifera resources. Further genetic relationship analysis discovered a high genetic similarity among wild populations, ranged from 0.700 3 to 0.940 9 while the cultivated population (KROMI) had an obvious genetic differentiation from wild populations.

中图分类号:

尚以顺,杨春燕, 钟理. 贵州野生匍匐剪股颖种质资源遗传多样性的ISSR分析[J]. 草业学报, 2009, 18(3): 67-73.

SHANG Yi-shun, YANG Chun-yan, ZHONG Li. Genetic diversity identification based on ISSR analysis of Agrostis stolonifera
germplasm resources in Guizhou[J]. Acta Prataculturae Sinica, 2009, 18(3): 67-73.

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