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草业学报 ›› 2009, Vol. 18 ›› Issue (5): 201-209.

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结缕草属植物种间关系和遗传多样性的SRAP标记分析

郭海林1,2,郑轶琦1,2,陈宣2, 薛丹丹1,2,刘建秀2*   

  1. 1.南京农业大学园艺学院,江苏 南京 210095;
    2.江苏省中国科学院植物研究所 南京中山植物园,江苏 南京 210014
  • 收稿日期:2008-09-16 出版日期:2009-10-20 发布日期:2009-10-20
  • 作者简介:郭海林(1975-),女,内蒙古乌盟人,助理研究员,在职博士生。E-mail: ghlnmg@sina.com
  • 基金资助:
    国家自然科学基金项目(30571307)资助

Genetic diversity and relationships of zoysiagrass as revealed by SRAP markers

GUO Hai-lin1,2, ZHENG Yi-qi1,2, CHEN Xuan2, XUE Dan-dan1,2, LIU Jian-xiu2   

  1. 1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
    2.Institute of Botany,
    Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
  • Received:2008-09-16 Online:2009-10-20 Published:2009-10-20

摘要: 通过SRAP标记技术对结缕草属植物5种1变种共96份种源进行遗传多样性分析。结果表明,1)结缕草属种质间存在丰富的遗传多样性,54对SRAP引物共扩增出362条清晰的用于多样性分析的谱带,其中多态性条带337条,多态性比率(PPB)为93.09%,Nei’s基因多样性指数(H)为0.240 9,Shannon信息指数(I)为0.374 6。2)应用Nei-Li相似系数法估算了96份材料间的遗传相似系数(GS),其GS为0.593 9~0.983 4,平均为0.758 8。不同种之间,结缕草与中华结缕草、沟叶结缕草与细叶结缕草中的部分材料遗传相似系数较高,遗传距离较近。大穗结缕草Z010和长花结缕草Z122与其他结缕草的遗传相似系数都相对较低。同一个种内,结缕草、中华结缕草和沟叶结缕草种内遗传变异均较大,GS分别为0.599 4~0.983 4,0.624 3~0.980 7和0.663 0~0.947 5。3)通过UPGMA分子系统聚类法,将96份种源分为7个大的类群,其中第1大类和第2大类均包含结缕草、中华结缕草和少量的沟叶结缕草种源;第3大类群主要包括4份美国引进的材料;第4大类主要包括沟叶结缕草和细叶结缕草;大穗结缕草Z010、长花结缕草Z122和结缕草Z115都单独聚为一类。从聚类结果可以看出,结缕草、中华结缕草和沟叶结缕草均有交叉聚类现象,并不是同一个种的材料完全相聚,个别种源自行一类,遗传基础与其他材料差异较大,从遗传聚类图可以很明确地看出96份种源间的遗传距离及亲缘关系。

Abstract: The genetic diversity and relationships of 98 germplasms of five species and one variety of Zoysia were analyzed using SRAP markers. A total of 362 bands were detected with 54 pairs of SRAP primers and 337 of the bands were polymorphic (PPB=93.09%). The Nei’s gene diversity index (H) was 0.240 9 and the Shannon diversity index (I) was 0.374 6, indicating a high level of genetic diversity of zoysiagrass. The genetic similarity (GS) among 96 Zoysia germplasms ranged from 0.593 9 to 0.983 4 with an average of 0.758. Eight showed that a large genetic polymorphism existed among the germplasms of Zoysia grass. The GS between Z. japonica and Z. sinica, and between Z. matrella and Z. tenuifolia were relatively high compared with the GS of other species. The variance ranges of GS within species of Z. japonica, Z. sinica and Z. matrella were 0.599 4-0.983 4, 0.624 3-0.980 7 and 0.663 0-0.947 5, respectively. Based on the presence of bands, 96 zoysia germplasm were classified into seven major groups by UPGMA cluster analysis, in which group I and group II included three species of Z. Japonica, Z. sinica and a few accessions of Z. matrella. Group III included four accessions introduced from America. Group IV mainly comsisted of Z. matrella and Z. tenuifolia. Z. machrostachya (Z010), Z .sinica var nipponica (Z122) and Z. sinica (Z115) formed a group by themselves. The Results of UPGMA cluster showed that some accessions of Z. japonica, Z. sinica and Z. matrella clustered into one group: no one species clustered into one group, Moreover, a very few groups contained only one accession, suggesting that it had a special genetic base. In this paper, the diversity and relationships among 96 germplasms have been investigated and a preliminary discussion made on the ownership of part accessions. This research should provide a scientific basis at the molecular level for further study and the application of Zoysia grass germplasms.

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