不同中草药复方制剂对云岭黑山羊生产性能及免疫功能的影响

摘要/Abstract

摘要： 本试验研究了日粮中添加不同中草药复方制剂对云岭黑山羊生产性能及免疫功能的影响。试验选用健康无病、出生日期和体重相近的云岭黑山羊成年母羊120只,随机分为4组,每组30只,其中1个对照组只饲喂基础日粮不添加制剂;3个试验组分别按精料的1.2%添加自制复方紫锥菊提取物、四君子散和强壮散。预试期为7 d,第1~2 天添加试验量的1/4,第3~4 天添加试验量的1/3,第5~6 天添加试验量的2/3,第7 天添加试验量(精料量的1.2%);正试期为60 d,分别于正试期第0,30,60 天清晨对所有试验羊进行空腹称重并准确记录,并测定其总增重和平均日增重,计算料重比,进而分析其经济效益;于试验结束当天从每组中分别随机选择10只羊进行空腹颈静脉采血,进行血液生理生化指标的测定。结果表明,1)复方紫锥菊提取物组云岭黑山羊成年母羊的总增重和平均日增重最高,分别为6.17 和0.103 kg,分别较对照组提高了17.97%和6.18%;2)复方紫锥菊提取物组的料重比最低为1.96∶1.00,分别比对照组、四君子散组、强壮散组降低了7.09%,17.76%,45.01%,且与强壮散组差异显著 (P＜0.05);3)复方紫锥菊提取物组羊的盈利额最大,每只羊每天可创效益4.25元,而对照组每只羊每天可创效益3.58元,比对照组提高了15.76%,而四君子散组、强壮散组的盈利额则低于对照组;4)复方紫锥菊提取物可显著提高血液中的红细胞数和红细胞压积(P＜0.05),对白细胞数也有一定的提高作用(P>0.05),白细胞数较对照组提高了3.98%,四君子散亦可显著提高血液中的红细胞数(P＜0.05);5)复方紫锥菊提取物和强壮散可显著提高血清总蛋白和球蛋白含量(P＜0.05),较对照组血清总蛋白分别提高了11.65%和12.33%,球蛋白分别提高了27.25%和22.16%;6)各试验组中胆固醇含量均有所降低(P>0.05),复方紫锥菊提取物、四君子散、强壮散组分别比对照组降低了15.25%,2.12%,6.78%;7)强壮散和复方紫锥菊提取物均可提高血清中免疫球蛋白G的含量,分别比对照组提高了3.5和1.3倍。综合研究表明,复方紫锥菊提取物对云岭黑山羊的生长有一定促进作用,并可增强其免疫功能,提高养羊的经济效益。

Abstract: Effect of different Chinese herbal compound on production performance and immune function of Yunling black goat was evaluated in this study. 120 adult ewes with close birth date and weight were randomly divided into 4 groups by 30 per group, in which one was chosed as the control group and the other 3 groups as treatments (adding 1.2% of concentrate with Echinacea extract compound, four gentlemen powder and strong powder in the feed, respectively). The experiment included preliminary trial period (7 d, 1/4 of trial amount was added in the first 2 d, 1/3 of it was added in the second 2 d, 2/3 of it was added in the third 2 d, all trial amount was added in the 7th day) and trial period (60 d). All adult ewes were weighed fastingly on the morning of the 0, 30th and 60th day, recorded the total gain, average daily gain, and calculated the feed conversion ratio and the economic effectiveness. 1) In groupⅠ, the total weight gain and average daily weight gain were both the highest, with the value of 6.17 and 0.103 kg, which were 17.97% and 6.18% higher than that of control group respectively, but those of other 2 treatments were both lower compared to the control group; 2) In groupⅠ, the feed conversion ratio was 1.96: 1, and it was lower than that of the control group and groupⅡ by 7.09%, 17.76% and significantly (P＜0.05) lower than that of group Ⅲ by 45.01%; 3) In groupⅠ, the net profit of sheep was the largest with the price of 4.25 Yuan/(d·ewe) which was 15.76% higher compared to the profit 3.58Yuan/(d·ewe) gained from the control group; 4)The number of red blood cell (RBC) and hematocrit (HCT) apparently increased in groupⅠ(P＜0.05), the number of white blood cell (WBC) was higher by 3.98% than that of the control group, and the number of RBC also increased greatly in groupⅡ(P＜0.05); 5) The serum total protein (TP) and globulin (GLB) of groupⅠand Ⅲ were significantly higher than those of the control group (P＜0.05), also the Serum TP was 11.65% and 12.33% higher than that of the control group respectively, and the GLB of the two groups increased by 27.25% and 22.16%; 6) The serum cholesterol (CHO) of the 3 treatments was less than that of the control group (P>0.05), and compared to the control group, the CHO content of groupⅠ,Ⅱand Ⅲ decreased by 15.25%, 2.12% and 6.78%, respectively; 7) In groupⅠand Ⅲ, the immunoglobulin G (IgG) content was higher than that of other groups, especially higher than that of the control group by 3.5 and 1.3 times respectively. In conclusion, the Echinacea extract compound can promote production performance and the immune function of Yunling black goat, and it also increases the economic benefit.

中图分类号:

刘志英,杨国荣,王春元,孙娟,郝智慧. 不同中草药复方制剂对云岭黑山羊生产性能及免疫功能的影响[J]. 草业学报, 2012, 21(3): 266-274.

LIU Zhi-ying, YANG Guo-rong, WANG Chun-yuan, SUN Juan, HAO Zhi-hui. Effect of different Chinese herbal compound on production performance
and immune function of Yunling black goat[J]. Acta Prataculturae Sinica, 2012, 21(3): 266-274.

参考文献

Xiao P G. International popular immunomodulator—Echinacea purpurea and its preparation. China Tradit Herb Drug,1996, 27(1): 46-48.
Liu Y B. Chemical and immune functions and clinic of preparations of Eehinacea purpurea. World Phytaned, 2001, 16(2): 47.
马小军, 王雅玲. 紫锥菊在北京地区的引种. 中国中药杂志, 1999, 24(10): 590-592.
肖培根. 国际流行的免疫调节剂一紫锥菊及其制剂. 中草药, 1996, 27(1): 462-481.
Bauer R, Wagner H. Potential immunostimulatory drugs. Economic and Medicinal Plant Research. New York: Academic Press, 1991.
Binns S E, Baum B R, Arnason J T. A taxonomic revision of Echinacea (Asteraceae: Heliantheae). Systematic Botany, 2002, 27(3): 610-632.
Sloley B D, Urichuk L J, Tywin C, et al. Comparison of chemical components and antioxidants capacity of different Echinacea species. Journal of Pharmacy and Pharmacology, 2001, 53(6): 849-857.
张英涛, 王弘, 艾铁民, 等. 松果菊属药用植物的应用基础研究. 北京大学学报, 2004, 36(1): 103-111.
倪耀娣, 钟秀会, 牛小飞, 等. 紫锥菊、黄芪提取物对鸡传染性法氏囊病疫苗免疫调节作用和生产性能的影响. 中国家禽, 2008, 30(16): 9-15.
王树琴, 郭世宁, 胡烨丹, 等. 紫锥菊对肉鸡体液免疫及细胞免疫的影响. 中国兽医杂志, 2008, 44(9): 48-49.
国家药典委员会. 中华人民共和国药典. 北京: 中国医药科技出版社, 2010.
王长彦, 杨鸣崎, 陈洪亮. 紫锥菊提取物的药理活性及在养殖业中的应用. 畜牧兽医杂志, 2008, 27(6): 69-71.
Wolfram G, Hanshelge M. A randomized controlled trial of the effect of fluid extract of Echinacea pururea on the incidence and severity of cold and respiratory infection. Clinical Studies, 1999, 106(2): 138-143.
云南省家畜家禽品种志编写委员会. 云南省家畜家禽品种志. 昆明: 云南科技出版社, 1987.
冷静, 杨俊霞, 杨舒黎, 等. 六种不同饲草对云岭黑山羊消化代谢的影响. 饲料工业, 2010, 31(23): 41-44.
杨胜. 家畜饲养实验指导. 北京: 农业出版社, 1990.
Zhang Y, Liu K, Wu L J. Advance in studies on Echinacea Moench. Chinese Traditional Herbal Drugs, 2001, 32(9): 852-855.
Soicke H, A1-Hassan G, Gorler K. Weitere kaffeesaure derivate aus Echinacea purpurea. Planta Medica, 1988, 54(2): 175-176.
谢仲权, 牛树琦. 天然物中草药饲料添加剂大全. 北京: 学苑出版社, 1996.
付秀花, 王盾, 顾宏伟. 中草药的免疫增强作用. 畜牧与兽医, 2002, (8): 40-43.
马得莹, 单安山. 中药对动物生长、内分泌和免疫功能的影响. 中国畜牧兽医, 2004, (1): 16-19.
高燕妮, 袁缨, 王吉磊, 等. 紫花松果菊提取物对肉仔鸡血清中抗氧化性能指标的影响. 中国畜牧兽医, 2008, 35(6): 18-21.
郝智慧, 陈杖榴, 邱梅, 等. 不同紫锥菊提取物对肉仔鸡免疫功能影响. 中兽医医药杂志, 2010, (2): 7-10.
任永林. 紫锥菊提取物对鲤鱼生长性能和免疫功能的影响. 雅安: 四川农业大学, 2008.
李佩国, 薛瑞晨. 动物生理学. 北京:中国农业科技出版社, 2000: 26-28.
武晓英, 曹贵东.纳米硒对岢岚绒山羊妊娠母羊血液生理生化指标的影响. 现代畜牧兽医, 2011, (6): 42-45.
李正本, 高桂生, 史秋梅, 等. 中草药免疫增强剂对鸡白细胞数量的影响. 河南农业科学, 2006, 6: 107-110.
周洪波, 操继跃. 中草药免疫促进剂的药效学研究进展. 中国兽药杂志, 2002, 36(6): 42-44.
程朝相, 张春杰, 李银聚, 等. 中药免疫增强剂对仔鸡免疫器官生长发育及免疫活性细胞影响的研究. 中国兽医杂志, 2002, 108(3): 6-8.
吕建存, 弓素梅, 王红芹. 四君子散对围产期奶牛免疫功能的影响. 湖北农业科学, 2010, 49(4): 919-921.
李宏权, 刘迎春, 宋建德, 等. 动物的红细胞免疫功能研究概述. 山西农业大学学报, 2002, 22(3): 269-273.
李江. 断乳仔兔纤维性腹泻发生机制及营养调控的研究. 保定: 河北农业大学, 2003.
周顺伍. 动物生物化学. 北京: 中国农业出版社, 2000.
Claudia B, Harald H, Goivanni L R, et al. Small intestinal morphology in eight-day-old calves fed colostrum for different durations or only milk replacer and treated with Long-R3-insulin growth factor I and growth hormone. Journal of Animal Science, 1998, 76 (3): 758-766.
Ahmed A F, Consatble P D, Misk N A. Effect of feeding frequency and route of administration on abomasal luminal pH in dairy calves fed milk replacer. Journal of Dairy Science, 2002, 85(6): 1502-1509.
刘晓琳, 郭世宁, 石达友, 等. 复方紫锥菊对肉鸡部分血液生化指标的影响. 中兽医医药杂志, 2008, (4): 30-32.
徐建忠, 邓鑫鑫, 史秋梅, 等. 紫黄散对人工感染传染性法氏囊病雏鸡血液生化指标的影响. 动物医学进展, 2010, 31(6): 53-57.
周振峰, 王晶, 王加启, 等. 裹包TMR饲喂对泌乳中期奶牛生产性能、养分表观消化率及血液生化指标的影响. 草业学报, 2010, 19(5): 31-37.
姜宁, 张爱忠, 李玲玲, 等.蝇蛆肤对大鼠机体抗氧化和免疫指标的影响. 动物营养学报, 2009, 21(4): 561-566.
吴华, Nardone A, Lacetera N. 紫锥菊提取物Polinacea^TM对乳牛外周血单核细胞免疫功能的影响.中国兽医科学, 2010, 40(5): 532-536.