两个高羊茅品种成熟种子再生体系的建立

草业学报 ›› 2011, Vol. 20 ›› Issue (2): 210-218.

两个高羊茅品种成熟种子再生体系的建立

闫军辉¹,赵智燕²,何亚丽^1*

1.上海交通大学农业与生物学院,上海 200240;
2.上海市南汇区绿化管理所,上海 201300

收稿日期:2010-03-02 出版日期:2011-02-25 发布日期:2011-04-20
通讯作者: E-mail:heyali@sjtu.edu.cn
作者简介:闫军辉(1985-), 男,河南项城人,在读硕士。E-mail:junhuiyan@sjtu.edu.cn
基金资助:
上海市农业委员会科技兴农重点攻关项目[沪农科攻字(2006)第4-5号]和国家科技部“十一五”科技支撑计划(2006BAD01A19-4-6)资助。

Tissue culture and plant regeneration from mature seed of two cultivars of tall fescue

YAN Jun-hui¹, ZHAO Zhi-yan², HE Ya-li¹

1.College of Agriculture and Biology Science, Shanghai Jiao Tong University, Shanghai 200240, China;
2.Landscaping Station of Nanhui District, Shanghai 201300, China

Received:2010-03-02 Online:2011-02-25 Published:2011-04-20

摘要/Abstract

摘要： 以2个高羊茅品种(“Plantation”和“沪坪1号”)的成熟种子为外植体,在MS培养基上分别添加不同浓度的2种植物生长调节剂,探索其愈伤组织的诱导、继代和分化的最佳激素配比及其相互间的影响,以建立简便高效的再生体系。结果表明,完整高羊茅种子最佳的愈伤诱导培养基为MS+2,4-D 3 mg/L,诱导率为71%;而把成熟种子进行纵切后得到的半粒种子的较优的愈伤诱导培养基为MS+2,4-D 2.5 mg/L,诱导率占半粒种子外植体的60%,占完整种子(2个半粒种子)外植体的90%以上;在最优诱导培养基上产生的愈伤组织的最佳继代培养基为MS+2,4-D 3 mg/L,其胚性愈伤诱导率为70%,而添加6-BA则抑制2,4-D对胚性愈伤组织的诱导;不同品种的最优的分化培养基不同——“Plantation”的最优分化培养基为MS+2,4-D 1 mg/L+6-BA 1 mg/L,而“沪坪1号”的最佳组合是MS+2,4-D 2 mg/L+6-BA 1 mg/L,分化率分别达到50%和56%。生根培养基采用已报道的组合1/2MS+NAA 0.5 mg/L,生根率达100%。

Abstract: To obtain a reliable and efficient regeneration system of tall fescue (Festuca arundinacea), mature seeds of ‘SHED’ and ‘Plantation’ were cultured on medium supplemented with combinations of 6-BA and 2,4-D. The optimal induction medium for intact mature seed explants was ‘MS+2,4-D 3 mg/L’, resulting in the highest induction rate of 71%, while that for longitudinally sliced seed explants was ‘MS+2,4-D 2.5 mg/L’ with a highest induction rate of 56%. The optimal subculture medium contained 3 mg/L 2,4-D, giving an embryogenic callus induction rate of 70%, and the addition of 6-BA to this medium adversely affected embryogenic callus induction. The effect of interaction of genotype and plant growth regulator concentration was significant. The highest regeneration rates obtained from the medium ‘MS+2,4-D 1 mg/L+6-BA 1 mg/L’ and ‘MS+2, 4-D 2 mg/L+6-BA 1 mg/L’ were 50% (CV. ‘SHED’) and 56% (CV. ‘Plantation’). The reported rooting medium of ‘1/2 MS+NAA 0.5 mg/L’ gave a rooting ratio of 100%.

中图分类号:

闫军辉,赵智燕,何亚丽. 两个高羊茅品种成熟种子再生体系的建立[J]. 草业学报, 2011, 20(2): 210-218.

YAN Jun-hui, ZHAO Zhi-yan, HE Ya-li. Tissue culture and plant regeneration from mature seed of two cultivars of tall fescue[J]. Acta Prataculturae Sinica, 2011, 20(2): 210-218.

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