Trehalose alleviates the negative effects of salinity on the growth and physiological characteristics of maize seedlings

doi:10.11686/cyxb2017156

Abstract

Abstract: The aim of this study was to investigate the effects of exogenous trehalose on the growth of maize seedings under salt stress. We evaluated the plant biomass, chlorophyll content, membrane lipid peroxidation, antioxidant enzyme activities, and contents of osmotic adjustment substances in maize seedlings (variety Zhengdan 958) in the control (no NaCl) and under salinity stress (150 mmol/L NaCl) with and without trehalose treatment. The results showed that, compared with the control, salt-stressed seedlings showed significantly lower dry weight, fresh weight, leaf area, chlorophyll content, and antioxidant enzyme activities, and significantly higher malondialdehyde (MDA) content, proline (Pro) content, soluble protein content, and membrane permeability. Among the salt-stressed maize seedlings, those treated with 10 mmol/L trehalose showed significantly increased biomass and chlorophyll content, decreased MDA content, Pro content, and membrane permeability, increased superoxide dismutase (SOD) and peroxidase (POD) activities, and increased soluble protein contents. These results indicated that exogenous trehalose could alleviate the negative effects of salinity stress on maize seedlings, and increase the resistance of maize seedlings to salinity stress.

TIAN Li-Xin, QU Dan-Yang, BI Wen-Shuang, XIE Teng-Long, LI Jing. Trehalose alleviates the negative effects of salinity on the growth and physiological characteristics of maize seedlings[J]. Acta Prataculturae Sinica, 2017, 26(8): 131-138.

References

[1] Sun Y L, Xu Y C, Li S, et al . Research progress on regulatory mechanisms of maize in response to salinity stress. Shandong Agricultural Sciences, 2016, 48(11): 157-163.
孙验玲, 徐远超, 李帅, 等. 玉米耐受盐胁迫的调控机理研究进展. 山东农业科学, 2016, 48(11): 157-163.
[2] Manzoor K, Ilyas N, Batool N, et al . Effect of salicylic acid on the growth and physiological characteristics of maize under stress conditions. Journal-Chemical Society of Pakistan, 2015, 37(3): 587-593.
[3] Zhang J L, Li H R, Guo S Y, et al . Research advances in higher plant adaptation to salt stress. Acta Prataculturae Sinica, 2015, 24(12): 220-236.
张金林, 李惠茹, 郭姝媛, 等. 高等植物适应盐逆境研究进展. 草业学报, 2015, 24(12): 220-236.
[4] Zhang H, Dong S T. Research progress on the physiological and biochemistry responses of salt tolerance and strategies of salt resistance in maize. Maize Science, 2011, 19(1): 64-69.
张红, 董树亭. 玉米对盐胁迫的生理响应及抗盐策略研究进展. 玉米科学, 2011, 19(1): 64-69.
[5] Zhang Y, Yang K J, Zhao C J, et al . Alleviation of the adverse effects of salt stress by regulating photosynthetic system and active oxygen metabolism in maize seedlings. Scientia Agricultura Sinica, 2014, 47(20): 3962-3972.
赵莹, 杨克军, 赵长江, 等. 外源糖调控玉米光合系统和活性氧代谢缓解盐胁迫. 中国农业科学, 2014, 47(20): 3962-3972.
[6] Xue Y W, Wang Y F, Yang K J, et al . Effects of exogenous nitric oxide on growth and osmoregulatory capability of maize ( Zea mays L.) seedlings under salt stress. Agricultural Research in the Arid Areas, 2016, 34(2): 171-176, 200.
薛盈文, 王玉凤, 杨克军, 等. 外源NO对NaCl胁迫下玉米幼苗生长和渗透调节能力的影响. 干旱地区农业研究, 2016, 34(2): 171-176, 200.
[7] Goddijn O, Verwoerd T C, Voogd E, et al . Inhibition of trehalase activity enhances trehalose accumulation in transgenic. Plant Physiology, 1997, 113(1): 181-190.
[8] Gerats T, Montagu M V, Caplan A B, et al . Effects of osmoprotectants upon NaCl stress in rice. Plant Physiology, 1997, 115(1): 159-169.
[9] Tarek E B, Haluk H, Hüseyin Ö A, et al . Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions. Plant Science, 2005, 169(1): 47-54.
[10] Fernandez O, Bethencourt L, Quero A, et al . Trehalose and plant stress responses: friend or foe. Trends Plant Science, 2010, (15): 409-417.
[11] Liu J, Jiang D G. Research advance on trehalose synthesis related genes in crops. Genomics and Applied Biology, 2014, 33(2): 432-437.
刘姣, 姜大刚. 作物海藻糖合成相关基因的研究进展. 基因组学与应用生物学, 2014, 33(2): 432-437.
[12] Xie D W, Wang X N, Fu L S, et al . Effect of exogenous trehalose on germ length and seeding freeze resistance of wheat under cold stress. Journal of Triticeae Crops, 2015, 35(2): 215-223.
谢冬微, 王晓楠, 付连双, 等. 外源海藻糖对冬小麦低温下胚芽长及幼苗抗寒性的影响. 麦类作物学报, 2015, 35(2): 215-223.
[13] Ali Q, Ashraf M, Anwar F, et al . Trehalose-induced changes in seed oil composition and antioxidant potential of maize grown under drought stress. Journal of the American Oil Chemists’ Society, 2012, 89(8): 1485-1493.
[14] Nounjan N, Teerakulpisut P. Effects of exogenous proline and trehalose on physiological responses in rice seedlings during salt-stress and after recovery. Plant Soil & Environment, 2012, 58(7): 309-315.
[15] Ding S H, Li Y Y, Wang B S. Effect of exogenous trehalose on salt tolerance of wheat seedlings. Acta Botanica Boreali-occidentalia Sinica, 2005, 25(3): 513-518.
丁顺华, 李艳艳, 王宝山. 外源海藻糖对小麦幼苗耐盐性的影响. 西北植物学报, 2005, 25(3): 513-518.
[16] Xu T, Zhou C Y, Zhou C, et al . Effect of trehalose on antioxidant system of melon seeding under salt stress. Northern Horticulture, 2014, (19): 28-30.
徐婷, 周传余, 周超, 等. 海藻糖对盐胁迫下薄皮甜瓜幼苗抗氧化系统的影响. 北方园艺, 2014, (19): 28-30.
[17] Abdallah M S, Abdelgawad Z A, El-Bassiouny H M S. Alleviation of the adverse effects of salinity stress using trehalose in two rice varieties. South African Journal of Botany, 2016, 103: 275-282.
[18] Yang L, Zhao X, Zhu H, et al . Exogenous trehalose largely alleviates ionic unbalance, ROS burst, and PCD occurrence induced by high salinity in Arabidopsis seedlings. Frontiers in Plant Science, 2014, 5: 1-11.
[19] Zhang Z L, Zhai W J. Plant Physiology Experiment Guide[M]. Beijing: Higher Education Press, 2003.
张志良, 翟伟菁. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2003.
[20] Giannopolitis C N, Ries S K. Superoxide dismutase. I. occurrence in higher plants. Plant Physiology, 1977, 59: 309-314.
[21] Hao Z B, Cang J, Xu Z. Plant Physiology Experiment[M]. Harbin: Harbin Institute of Technology Press, 2004.
郝再彬, 苍晶, 徐仲. 植物生理实验[M]. 哈尔滨: 哈尔滨工业大学出版社, 2004.
[22] Carl N, Ayah D. Snapshot: reactive oxygen intermediates. Cell, 2010, 140: 952.
[23] Yang S Y, Chen X Y, Hui W K, et al . Progress in responses of antioxidant enzyme systems in plant to environmental stresses. Journal of Fujian Agriculture and Forestry University: Natural Science Edition, 2016, 45(5): 481-489.
杨舒贻, 陈晓阳, 惠文凯, 等. 逆境胁迫下植物抗氧化酶系统响应研究进展. 福建农林大学学报: 自然科学版, 2016, 45(5): 481-489.
[24] Wang L Y, Zhao K F. Some physiological response of Zea mays under salt-stress. Acta Agronomica Sinica, 2005, 31(2): 264-266.
王丽燕, 赵可夫. 玉米幼苗对盐胁迫的生理响应. 作物学报, 2005, 31(2): 264-266.
[25] Abebe T, Guenzi A C, Martin B, et al . Tolerance of mannitol-accumulating wheat to water stress and salinity. Plant Physiology, 2003, 131(4): 1748-1755.
[26] Alqarawi A A, Allah E F A, Hashem A. Alleviation of salt-induced adverse impact via mycorrhizal fungi in Ephedra aphylla Forssk. Journal of Plant Interactions, 2014, 9(1): 802-810.
[27] Theerakulp P, Phongngarm S. Alleviation of adverse effects of salt stress on rice seedlings by exogenous trehalose. Asian Journal of Crop Science, 2013, 5(4): 405-415.
[28] Qin X F, Gao Y F, Lv W Y. Effects of NaCl stress on the seed germination and the seeding growth of four kinds of corn varieties. Seed, 2007, 26(5): 24-26.
秦雪峰, 高扬帆, 吕文彦. NaCl胁迫对玉米种子萌发和幼苗生长的影响. 种子, 2007, 26(5): 24-26.
[29] Wang F, Chang P P, Chen Y P, et al . Effect of exogenous nitric oxide on seeding growth and physiological characteristics of maize seedings under cadmium stress. Acta Prataculturae Sinica, 2013, 22(2): 178-186.
王芳, 常盼盼, 陈永平, 等. 外源NO对镉胁迫下玉米幼苗生长和生理特性的影响. 草业学报, 2013, 22(2): 178-186.
[30] Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 2002, 7(9): 405-410.
[31] Yan D L, Zheng B S. Effects of soaking seeds in trehalose on physiological characteristics of wheat Yangmai-19 under salt stress. Acta Agriculturae Zhejiangensis, 2016, 28(8): 1271-1276.
闫道良, 郑炳松. 海藻糖浸种对盐胁迫下扬麦19生理特性的影响. 浙江农业学报, 2016, 28(8): 1271-1276.
[32] Theerakulpisut P, Gunnula W. Exogenous sorbitol and trehalose mitigated salt stress damage in salt-sensitive but not salt-tolerant rice seedlings. Asian Journal of Crop Science, 2012, 4(4): 165-170.
[33] Chen S L, Peng Y, Zhou H, et al . Research advances in trehalose metabolism and trehalose-6-phosphate signaling in plants. Plant Physiology Journal, 2014, 50(3): 233-242.
陈素丽, 彭瑜, 周华, 等. 植物海藻糖代谢及海藻糖-6-磷酸信号研究进展. 植物生理学报, 2014, 50(3): 233-242.
[34] Liu J X, Wang X, Li B P. Effects of exogenous nitric oxide donor SNP on ascorbate-glutathione cycle metabolism in ryegrass seedling leaves under NaCl stress. Acta Prataculturae Sinica, 2010, 19(2): 82-88.
刘建新, 王鑫, 李博萍. 外源一氧化氮供体SNP对NaCl胁迫下黑麦草幼苗叶片抗坏血酸-谷胱甘肽循环的影响. 草业学报, 2010, 19(2): 82-88.
[35] Wang L X, Zhao Z G, Wang S M. Effect of nitric oxide on metabolism of reactive oxygen species and membrane lipid peroxidation in Triticum aestivum leaves under water stress. Acta Prataculturae Sinica, 2006, 15(4): 104-108.
王罗霞, 赵志光, 王锁民. 一氧化氮对水分胁迫下小麦叶片活性氧代谢及膜脂过氧化的影响. 草业学报, 2006, 15(4): 104-108.
[36] Tian X Y, Liu Y J, Guo Y C. Effect of salt stress on Na + , K + , proline, soluble sugar and protein of NHC. Pratacultural Science, 2008, 25(10): 34-38.
田晓艳, 刘延吉, 郭迎春. 盐胁迫对NHC牧草Na + 、K + 、Pro、可溶性糖及可溶性蛋白的影响. 草业科学, 2008, 25(10): 34-38.
[37] Wang N, Cao M J, Yu J L. Effects of NaCl stress on organic osmotica of maize seedlings. Maize Science, 2009, 17(4): 61-65, 69.
王宁, 曹敏建, 于佳林. NaCl胁迫对玉米幼苗有机渗透调节物质的影响. 玉米科学, 2009, 17(4): 61-65, 69.
[38] Du J, Xiang C Y, Zhang H Y, et al . Effects of NaCl stress on osmosis-regulating substance and Na + , K + , Ca 2+ contents of maize seedling. Seed, 2014, 33(7): 19-23.
杜锦, 向春阳, 张红颖, 等. NaCl胁迫对玉米幼苗渗透调节物质及Na + 、K + 、Ca 2+ 含量的影响. 种子, 2014, 33(7): 19-23.
[39] Wang S G, Deng R F, Zhuang Y, et al . Effects of trehalose pretreatments on salt resistance in wheat seedings. Journal of Southwest Agricultural University, 1992, 14(2): 182-185.
王三根, 邓如福, 装炎, 等. 海藻糖提高绵阳15号小麦幼苗耐盐能力的研究. 西南农业大学学报, 1992, 14(2): 182-185.