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Acta Prataculturae Sinica ›› 2017, Vol. 26 ›› Issue (8): 216-222.DOI: 10.11686/cyxb2016436

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Nitrogen and phosphorus ecological stoichiometry in different-aged alfalfa stands in southeast Shandong

WANG Hui1, **, MIAO Fu-Hong1, **, SUN Juan1, LIU Hong-Qing2, YANG Guo-Feng2, *   

  1. 1.College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China;
    2. College of Life Science, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2016-11-22 Online:2017-08-20 Published:2017-08-20

Abstract: The main aim of this study was to explore the stoichiometry of nitrogen (N) and phosphorus (P) in alfalfa stands of different ages and cutting frequencies in the southeast Shandong area. We analyzed N and P contents and their stoichiometric ratios in the leaf and stem of alfalfa plants in stands of different ages (2, 3, 4, and 5 years). The leaf and stem N and P contents of alfalfa differed significantly among different stand ages and cutting frequencies. The leaf and stem N and P contents were higher in the 3-year-old alfalfa stand than in alfalfa stands of other ages. The leaf and stem N∶P ratios were highest in the 4-year-old alfalfa stand and lowest in the 3-year-old alfalfa stand. The leaf and stem N and P contents of alfalfa first increased and then decreased with increased cutting frequency, reaching a maximum at the third cut (except the stem N content of 3-year-old alfalfa). The leaf N∶P ratio in 2-year-old and 4-year-old alfalfa stands first decreased and then increased with cutting, while the stem N∶P ratio first decreased and then increased with cutting (except in the 5-year-old stand), and showed the minimum value at the fourth cut. The N and P contents and the N∶P ratios in the leaf and stem were affected significantly by stand age and the cutting frequency. The N contents in the leaf and stem were positively correlated with the N∶P ratio. The P contents in the leaf and stem were negatively correlated with the N∶P ratio. These results show that the nutrient limiting alfalfa growth shifted from N to P as the stands aged.