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Acta Prataculturae Sinica ›› 2017, Vol. 26 ›› Issue (10): 158-169.DOI: 10.11686/cyxb2017009

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Cultural characteristics of fungal endophyte from 3 locoweed species in China

WANG Zhi-Gang, CAO Shi, XU Na, XU Shan, LI Yan-Zhong*   

  1. State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
  • Received:2017-01-09 Online:2017-10-20 Published:2017-10-20

Abstract: Locoweeds contain swainsonine-producing fungal endophytes. Endophytes in several locoweed species in China are known to belong to the same taxonomic species of fungus. However, since these endophytes are isolated within their respective hosts and vertically transmitted to seed of the next plant generation, it is likely that the fungal endophytes of the various locoweed species have developed distinctive biological traits. Colony growth rate and morphological characteristics of endophytes from Oxytropis glabra, O. ochrocephala and O. kansuensis (strains LYZ0091, LYZ0093 and LYZ0109, respectively) were studied under different temperature and pH regimes and grown on various media to test this hypothesis. It was found that: 1) The maximum temperature for growth differed between strains (LYZ0109 ceased growth at 30 ℃, while LYZ0091 and LYZ0093 continued to grow at 30 ℃); 2) The optimum temperatures for colony growth were 20, 25 and 25 ℃, respectively, for LYZ0091, LYZ0093, and LYZ0109; 3) The growth rate of LYZ0091 at 5, 10 and 15 ℃ was significantly (P<0.05) higher than LYZ0109, while significantly (P<0.05) lower than LYZ0109 at 20 and 25 ℃. 4) No significant growth difference between fungal strains was found over the pH range pH 4 to pH 11; 5) The colony growth rate of strain LYZ0109 was significantly (P<0.05) higher than that of LYZ0091 on NA, PCA, PDA, PSA and WHDA media for most growth measures; 6) No conidia were produced by any fungal strain in any of temperature, pH and media combinations tested; 7) Hyphae of LYZ0091 and LYZ0109 were waved, and seldom produced chlamydospores; hyphae of LYZ093 were straight with plentiful chlamydospores arranged as tree-like structures from the media bottom to the surface.